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  Research Article

  Genetic Regulation of the Bacterial Omega-3 Polyunsaturated Fatty Acid Biosynthesis Pathway

  The production of long-chain omega-3 polyunsaturated fatty acids (PUFA) by marine shadowrocket使用方法, particularly those from deep-sea environments, has been known for decades. These unique fatty acids are produced by a polyketide-type mechanism and subsequently incorporated into the phospholipid membrane. While much research has focused on the biosynthesis genes, their products, and the phylogenetic distribution of these gene...

  Marco N. Allemann, Eric E. Allen
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  Research Article

  Enzymatic and Mutational Analysis of the PruA Pteridine Reductase Required for Pterin-Dependent Control of Biofilm Formation in Agrobacterium tumefaciens

  Biofilms are complex multicellular communities that are formed by diverse bacteria. In the plant pathogen Agrobacterium tumefaciens, the transition from a free-living motile state to a nonmotile biofilm state is governed by a novel signaling pathway involving small molecules called pterins. The involvement of pterins in biofilm formation is unexpected and prompts many...

  Monica Labine, Lisa DePledge, Nathan Feirer, Jennifer Greenwich, Clay Fuqua, Kylie D. Allen
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  Research Article

  Molecular Design of a Signaling System Influences Noise in Protein Abundance under Acid Stress in Different Gammaproteobacteria

  Acid resistance is an important property for bacteria, such as Escherichia coli, to survive acidic environments like the human gastrointestinal tract. E. coli possesses both passive and inducible acid resistance systems to counteract acidic environments. Thus,...

  Sophie Brameyer, Elisabeth Hoyer, shadowrocket使用方法, Korinna Burdack, shadowsock下载 ios, Kirsten Jung
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  Research Article | Spotlight

  The Length of Lipoteichoic Acid Polymers Controls Staphylococcus aureus Cell Size and Envelope Integrity

  Methicillin-resistant Staphylococcus aureus (MRSA) is a common cause of community- and hospital-acquired infections and is responsible for a large fraction of deaths caused by antibiotic-resistant bacteria. S. aureus is surrounded by a complex cell envelope that protects it from antimicrobial...

  Anthony R. Hesser, Leigh M. Matano, Christopher R. Vickery, B. McKay Wood, Ace George Santiago, Heidi G. Morris, Truc Do, Richard Losick, Suzanne Walker
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  Research Article

  The C Terminus of Substrates Is Critical but Not Sufficient for Their Degradation by the Pseudomonas aeruginosa CtpA Protease

  Carboxyl-terminal processing proteases (CTPs) are found in all three domains of life, but exactly how they work is poorly understood, including how they recognize substrates. Bacterial CTPs have been associated with virulence, including CtpA of Pseudomonas aeruginosa, which works in complex with the outer membrane lipoprotein LbcA to degrade potentially dangerous...

  Sammi Chung, Andrew J. Darwin
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  Research Article

  Two Interacting ATPases Protect Mycobacterium tuberculosis from Glycerol and Nitric Oxide Toxicity

  A better understanding of the pathogenesis of tuberculosis requires a better understanding of gene function in M. tuberculosis. This work provides the first functional insight into the Rv3679/Rv3680 ATPase complex. We demonstrate that M. tuberculosis requires this complex and specifically its ATPase...

  Meredith Whitaker, Nadine Ruecker, Travis Hartman, Thais Klevorn, Jaclynn Andres, Jia Kim, Kyu Rhee, Sabine Ehrt
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  Research Article

  An ECF41 Family σ Factor Controls Motility and Biogenesis of Lateral Flagella in Azospirillum brasilense Sp245

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  Ashutosh Prakash Dubey, Parul Pandey, Vijay Shankar Singh, Mukti Nath Mishra, Sudhir Singh, Rajeev Mishra, Anil Kumar Tripathi
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  shadowrocket使用方法

  Assembly Mechanism of a Supramolecular MS-Ring Complex To Initiate Bacterial Flagellar Biogenesis in Vibrio Species

  Flagellar formation is initiated by the assembly of the FliF protein into the MS-ring complex, which is embedded in the cytoplasmic membrane. The appropriate spatial/temporal control of MS-ring formation is important for the morphogenesis of the bacterial flagellum. Here, we focus on the assembly mechanism of Vibrio FliF into the MS-ring. FlhF, a positive regulator of the number and location of flagella, recruits the FliF...

  Hiroyuki Terashima, Keiichi Hirano, Yuna Inoue, Takaya Tokano, Akihiro Kawamoto, Takayuki Kato, Erika Yamaguchi, Keiichi Namba, Takayuki Uchihashi, Seiji Kojima, Michio Homma
• Open Access

  

  Research Article | Spotlight

  Functional Determinants of a Small Protein Controlling a Broadly Conserved Bacterial Sensor Kinase

  One of the primary means by which bacteria adapt to their environment is through pairs of proteins consisting of a sensor and a response regulator. A small membrane protein, MgrB, impedes the activity of sensor protein PhoQ, thereby affecting the expression of PhoQ regulated virulence genes in pathogenic bacteria. However, it is unknown how such a small protein modulates the activity of PhoQ. Here, we studied the functional determinants...

  Srujana S. Yadavalli, Ted Goh, Jeffrey N. Carey, Gabriele Malengo, Sangeevan Vellappan, Bryce E. Nickels, Victor Sourjik, Mark Goulian, Jing Yuan
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  Research Article

  Genetic Control of Oxidative Mutagenesis in Sulfolobus acidocaldarius

  Hyperthermophilic archaea are expected to have effective (and perhaps atypical) mechanisms to limit the genetic consequences of DNA damage, but few gene products have been demonstrated to have genome-preserving functions in vivo. This study confirmed by genetic criteria that the S. acidocaldarius Ogg protein avoids the characteristic mutagenesis of G...

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